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          全球領先的表面張力儀測量技術——精確快速測量動靜態表面張力

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          應用不同組裝的磷脂酰膽堿對牛精漿蛋白的隔離:一種新的技術方法——結論、致謝!

          來源:上海謂載 瀏覽 163 次 發布時間:2021-12-20

          5.結論


          首先,表面張力測量證明牛精漿具有很好的表面活性,可能與BSP蛋白有關。其次,Langmuir膜法(先前被證明是公牛精子外細胞膜的相關脂質單層模型[35])有助于表征BSP蛋白對脂質膜的親和力。BSP蛋白能夠到達被磷脂覆蓋的牛精子細胞表面,這得益于它們與磷脂酰膽堿的相互作用和自身的表面活性。我們的假設是,由于它們自身的表面活性,它們首先穿透精子的外小葉,然后由于與磷脂酰膽堿的強烈相互作用而留在那里。最后,利用Langmuir膜法對已知的BSP蛋白螯合劑如ldl和脂質體的作用進行了篩選。脂質體與低密度脂蛋白一樣有效地阻止BSP蛋白插入磷脂層。低密度脂蛋白和脂質體的比例相同:0.16–0.17 mg磷脂酰膽堿/mg BSP。我們還不知道在冷凍保存的時間尺度上脂質體的非常微弱的插入是否有一些生物學后果。需要進一步研究分析每種BSP蛋白的表面性質及其在膜上的作用。此外,可以測試Langmuir膜,以檢測BSP與膜的親和力隨溫度的變化[20]。最后,Langmuir膜法是一種有效的篩選BSP蛋白質新螯合劑的方法。該方法可適用于其他動物物種。


          致謝


          作者感謝IMV Technologies(法國)的財務支持,Gérard Chatagnon負責精漿蛋白質定量,Véronique Solé負責凝膠電泳,Maureen Collobert負責英語校正,Alain Sire和Patrice Papineau負責手套箱的構思和實現,允許在受控大氣中使用朗繆爾槽。


          附錄A.補充數據


          與本文相關的補充數據可在在線版本中找到,網址為http://dx.doi.org/10.1016/j.colsurfb.2015.11.34


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